Electron Spin Resonance Studies on the Damage of Ribonuclease Exposed to Ultra-violet Light

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概要

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• Primary damage in RNase caused by exposure to UV-light was studied by ESR. Frozen aqueous solution of RNase was irradiated by 254 mp light and ESR spectra and remaining enzymatic activity were measured. ESR spectra showed that the free radicals formed in RNase were localized in chromophores of RNase; namely tyrosine, phenylalanine and cystine. Effect of warming on the spectra suppored the assignments. The amount of radicals in RNase had linear relationship with the extent of enzyme inactivation.

• 日本放射線影響学会の論文

著者

• 沢田 文夫

  放射線医学総合研究所化学研究部

• 市村 幸子

  Division Of Chemistry National Institute Of Radiological Sciences

• 沢田 文夫

  Division of Chemistry, National Institute of Radiological Sciences

• 染野 和雄

  Goverment Chemical Industrial Research Institute

関連論文

• Characterization of Ribonuclease A Irradiated with γ-Rays in the Presence of Cytidylic Acid with Respect to the Interaction of the Enzyme with Folic Acid : SHORTC OMMUNICATION
• Biochemical Analyses of Some Metabolites in Urine and Blood in Persons Exposed Accidentally to a Source of ^Ir
• Electron Spin Resonance Studies on the Damage of Ribonuclease Exposed to Ultra-violet Light

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