Appearance of Low Molecular Weight DNA in a Rec- Mutant of Escherichia coli K 12 Irradiated with X-rays

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概要

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• A Rec^- mutant of Escherichia ccli K 12 (JC 1569 b) was irradiated with X-rays, lysed gently with lysozyme and centrifuged in alkaline sucrose gradients. The sedimentation profile for DNA demonstrated that X-rays produces low moleuclar weight DNA and that the sedimentation profile altered by irradiation with X-rays cannot be restored after incubation. In contrast, the altered profile for the original Rec^+ bacteria was restored almost completely under the same conditions. These results suggested that X-rays yields single-strand breaks in the DNA of Rec^- and of Rec^+ bacteria and that the break in the DNA of Rec^+ bacteria can be repaired but not the break in Rec^- bacteria.

• 日本放射線影響学会の論文

著者

• 森明 充興

  放射線医学総合研究所化学部

• 森明 充興

  Laboratory of Biochemistry, Division of Chemistry, National Institute of Radiological Sciences

• 堀井 善一郎

  On leave from the Department of Fundamental Radiology, Osaka University, School of Medicine

• 堀井 善一郎

  On Leave From The Department Of Fundamental Radiology Osaka University School Of Medicine

関連論文

• Identification of an Amber uvrC Mutant in Escherichia coli K-12
• Appearance of Low Molecular Weight DNA in a Rec- Mutant of Escherichia coli K 12 Irradiated with X-rays

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