Identification of an Amber uvrC Mutant in Escherichia coli K-12

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An Escherichia coli K-12 ultraviolet (UV)-sensitive mutant KMBL92 (dar-4) was identified as an amber uvrC mutant. In a suppressor-free genetic background it showed high sensitivity to UV, no reactivation of UV-irradiated λ phage and positive Weigle reactivation, whereas upon introduction of a suppressor mutation it exhibited moderate resistance to UV, inter-mediate capacity for host-cell reactivation and no Weigle reactivation. Other properties, like DNA degradation during post-UV incubation and frequencies of UV-induced mutations in bacteria and bacteriophage λ, were similar to those of a uurC mutant. There are four genes affecting high ultraviolet (UV)-sensitivity in excision repair; uvrA, uvrB, uvrC, and uvrD or uvrE. Among a series of UV-sensitive mutants isolated by van de Putte et al. (1965) from an Escherichia coli strain KMBL49 (dar+)1), KMBL90 (dar-1) and KMBL101 (dar-6) were mapped in the uvrB gene and showed typical properties of uvrB mutants; they were highly sensitive to UV and did not reactivate UV-irradiated phagel).
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