スブチリシンBPN′の熱および圧力変性機構(<特集>高圧力下の溶液)

概要

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The pressure effects on thermal inactivation of subtilisin BPN′(S-BPN′) and its pressure inactivation have been studied by measuring the rate of hydrolysis of succinyl-l-alanyl-l-alanyl-l-prolyl-l-phenylalanine-p-nitroanilide(SAAPFpNA) catalyzed by S-BPN′ in the temperature range of 15〜67.5℃ and at the pressures up to 700 MPa. The apparent rate of hydrolysis decreased with time above a certain temperature and pressure. The decrease is due to the decrease in S-BPN′ activity. The enthalpy change of the thermal inactivation was 100〜220 kJ・mol^<-1> and the volume change of this process was 18〜-4 cm^3・mol^<-1>. While S-BPN′ was inactivated in the pressure range of 500〜700 MPa at 15〜45℃, the corresponding volume change was -13〜-22 cm^3・mol^<-1>.(
社団法人日本材料学会の論文
1996-03-15