THE UPTAKE OF [^3H]ESTRADIOL BY MOUSE PREIM-PLANTAION EMBRYS in vitro
スポンサーリンク
概要
- 論文の詳細を見る
Mouse blastocysts collected on day 4 were cultured for 20 hours in media containing various concentrations of [^3H]estradiol with/without l0^<-6>M unlabelled estradiol. There was a significant reduction in the radioactivity of embryos cultured at 4℃ compared with that of embryos cultured at 37℃. The radioactivity of embryos kept in the washing solution after washing was slight lower but showed no significant difference when it was compared with that of embryos not kept in this way. There was no significant difference between the radioactivity of embryos cultured in 2 x 10^<-8>M [^3H]estradiol and that of embryos cultured in 2 x 10^&t;-8>M [^3H]estradiol plus 10^<-6>M nonlabelled estradiol. Furthermore the radioactivity of embryos incubated in 2.5xl0^<-8>M [^3H]estradiol is more than twice that of embryos incubated in l0^<-8>M [^3H]estradiol. These results demonstrate that preimplantation mouse embryos are capable of uptake and retaining of estrogen and thaf the mechanism of uptake is neither selective nor receptor mediated.
- 社団法人日本産科婦人科学会の論文
- 1985-01-01
著者
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Hoshiai Hiroshi
Reproductive Biology. Harvard Medical School. U. S.a.
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UEHARA Shigeki
Department of Obstetrics and Gynecology, Tohoku University School of Medicilee
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VILLEE Claude
Department of Reproductive Biochemistry, Laboratory of Human Reproduction
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UEHARA SHIGEKI
Tohoku Kousai Hospital
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Villee Claude
Department Of Reproductive Biochemistry Laboratory Of Human Reproduction
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Uehara Shigeki
Department Of Obstetrics And Gynecology Tohoku Kosai Hospital
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Uehara Shigeki
Department of Applied Chemistry, Yamanashi University
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