神経芽腫特異抗原の細胞免疫学的研究

概要

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Cytotoxic T lymphocytes (CTL) against syngeneic A/J mouse neuroblastoma (C-1300) were induced in vitro primary or secondary culture. Its specificity as CTL was characterized by treatment with monoclonal anti-Thy 1.2 or anti-Lyt 2.2 antibody and complement. CTL specific for syngeneic neuroblastoma C-1300 cells definitely cross reacted with human neuroblastoma cells (NB 1, NBGOTO) across a species barrier. In next experiment, using mixed lymphocyte tumor cell culture by 51^Cr release assays for 14 patients with various types of pediatric malignant solid tumor, the following results were obtaind. In peripheral blood lymphocytes of post operative neuroblastoma patients, CTL were induced in vitro against allogeneic neuroblastoma cell (NBGOTO), and in some of them, specific cytotoxic antivity for C-1300 were noticed. But no CTL could be detected from patients of preoperative, during therapy periods and with other malignant tumors. Surprisingly, in patients with Stage IV-S neuroblastoma, high level of CTL was appeared in each time, even in preoperative period. This result indicates that Stage IV-S neuroblastoma is associated with high levels of immunological surveillance. The conclusion is that a clearly cross activity exists in T cell mediated cytotoxic reaction between the mouse and human neuroblastoma without recognizing both MHC determinants.
1985-05-20