74 カキ培養シュートの発根促進に影響する果糖添加培地中の物質の特定

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In this experiment, the influence of medium components had on the root induction of in vitro propagated Japanese persimmon shoots, using a modified MS medium (1/2 nitrogen) containing fructose and 2g/l gellan gum as the gelling agent, was investigated. The medium supplemented with 0.2M fructose and 0.01M NH_4NO_3 had the highest value for frequency of rooting. Rooting percentages decreased as the fructose concentrations were lowered. When the medium supplemented with 0.01M NH_4NO_3 decreased in rooting frequency, it had lower concentrations of fructose compared to 0.0025M and 0.04M NH_4NO_3. Rooting was observed when gellan gum was used, however no rooting took place when glucose, rhamnose and (or) sodium glucronate were used instead of gellan gum. It appears that gellan gum enhances root induction as a polysaccharide in the medium.
植物化学調節学会の論文
2002-10-31