36 新規発根促進物質N-(フェネチル)コハク酸アミドの精製と単離

概要

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Root-promotin substance in industrially fermented broth of Bacillus sp. Were purified by using adzuki rooting test as an activity index. Four liters of the broth was desalted by polymer resin, and purified successively by anion exchange resin, acidic n-buthanol extraction and SepPak C18 cartridge. Active fraction was purified further by HPLC for five times to obtain single peak fraction. The yield was 3.2 mg. Based on the analytical data of mass, IR and NMR spectra, the chemical structure of this compound was elucidated as N-(phenethly)succinamic acid (PESA), and identified by using chemically synthesized PESA. Root-promoting activity of PWSA was increased linearly in the range of concentration from 0.01 mM to 1 mM, and the maximum number of protruded roots was substantially higher than that of indole-3-acetic acid.
植物化学調節学会の論文
1999-10-01