E-50 アスパラガスにおける高頻度不定胚形成およびその経路の多様性に関する研究

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A high frequency somatic embryogenesis system was established in Aspargus offcinalis L. Studies focused on pathways of somatic embryogenesis and factors to maximize somatic embryogenesis frequency. Calli were induced from terminal buds, hypocotyl and primary root of 2week-old seedlings, and subcultured in MS semisolid medium supplemented with 2mg/L 2, 4-D. Three types of calli were obtained: type I was whitish and almost composed of free single cells; type II was yellowish, friable and small granular; and type III was greenish, compact and large tubercular. Somatic embryogenesis cometence of these calli were and it was found that type I had no ability to from somatic embryo, type III produced few somatic embryos, on the contrary, type II exhibited the most high frequencyabout 90% of the cells or clusters could develop into somatic embryos. The optioize'd medium for Asparagus somatic embryogenesis was MS medium. MS medium compensated with both auxin and cytokinin was better than individual application. The highest frequency of somatic embryogenesis was obtained at 0.5mg/L 2, 4-D and 0.5-1mg/L BA. Sucrose at a concentration of 2% was the most effective one. It is of interest that the callus could also produce heart-shaped and mature embryoids with two cotyledons, and showed monocot, dicot and embryogenesis pathways, though Asparagus is monocot. This unque embryological evidence confirms that monocot was derived from dicot.
植物化学調節学会の論文
1998-10-01

E-50 アスパラガスにおける高頻度不定胚形成およびその経路の多様性に関する研究

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