COLLABORATIVE WORK TO EVALUATE TOXICITY ON MALE REPRODUCTIVE ORGANS BY REPEATED DOSE STUDIES IN RATS : 1)EFFECTS OF 2-WEEKS DAILY ADMINISTRATION OF ESTRADIOL BENZOATE

概要

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As part of a collaborative project to assess whether a 2-weeks administration period is sufficient to detect testicular toxicity of various compounds, male rats were subcutaneously administered 0, 5, 20, 50 or 100μg/kg of estradiol benzoate (E_2B), a known testicular toxicant, daily for 2 or 4 weeks. After 4-weeks, suppression of body weight gain, increase in the weight of the adrenal gland, and gross changes such as decrease in size of the prostate and seminal vesicles, and increase in size of the adrenal gland were observed in the 5, 20, 50 and 100μg/kg groups. On histopathological examination, degeneration/necrosis of Pachytene spermatocytes, atrophy of Leydig cells, mature spermatid retention (Lee, et al. , 1993) at stages IX, X and XI, and atrophy of ducts of the epididymides were also observed in the 5, 20, 50 and 100μg/kg groups After 2-weeks, the same changes were also observed with 20, 50 and 100μg/kg, but not 5μg/kg. These results indicate that the toxic effects of E_2B are detectable by administration for 2 weeks at an appropriate dose level.
日本トキシコロジー学会の論文
2000-10-25