Seed Abortion in Crosses between Diploid and Tetraploid Grapes (Vitis vinifera and V. complex) and Recovery of Triploid Plants through Embryo Culture
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概要
- 論文の詳細を見る
Interploid crosses between two diploid and two tetrapkiod grape cultivars were carried out to produce abortive seeds with underdeveloped triploid embryos. Growth regulations influencing the recoveryof triploid plants from the underdeveloped embryos were individually examined in vitro using MS medium. Of six growth regulations added to the medium, malt extract (25-1600mg/l), casein hydrolysate (20-1500mg/l), BA (0.01-1.25mg.l) and GA[3] (0.1-1mg/l) did not show obvious effect on the recovery, whereas IAA and NAA showed small promotiveeffect at the concentration of 0.25 and 1.25mg.l and 0.01mg/l respectively. Secondly, 23 interploid crosses with four diploid and hour tetraploid grape cultivars were carried out reciprocally to produce triploid plants through embryo culture using MS medium supplemented with 1mg/l GA[3] and 100mg/l casein hydrolysate. In the 2x*4x crosses, 50 triploid seedlings were successfully recovered from 7.3% of inderdeveloped embryos culutured, while 33 seedlings were recovered from 2.8% of those in the reciprocals. Of the total of 88 seedling recovered, 38 were obtained through secondary embryo formation in vitro. From these results, it was suggested that the production rates of triploid plants from the interploid crosses increase with the aid of embryo culture, and that in this case in vitro formation of secondary embryos is an important key factor to increase the rates.
- 九州大学の論文
著者
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Wakana Akira
Laboratory of Agricultural Ecology,Division of Plant Resources,Graduate School of Bioresource and Bi
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Park Sung
Laboratory of Agricultural Ecology,Division of Plant Resources,Graduate School of Bioresource and Bi
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Park S
江原大
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Wakana A
Kyushu Univ. Fukuoka Jpn
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Wakana Akira
Laboratory Of Agricultural Ecology Division Of Plant Resources Graduate School Of Bioresource And Bi
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Park Sung
Laboratory Of Agricultural Ecology Division Of Plant Resources Graduate School Of Bioresource And Bi
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Hiramatsu Michikazu
Laboratory of Horticultural Science,Division of Agricultural Botany,Department of Plant Resources,Fa
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Hiramatsu Michikazu
Laboratory Of Horticultural Science Division Of Agricultural Botany Department Of Plant Resoruces Fa
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Hiramatsu Michikazu
Fruit Science Laboratory Faculty Of Agriculture Kyushu University
関連論文
- Pollen tube behaviors in self-incompatible and self-compatible Citrus cultivars
- Seed Abortion in Crosses between Diploid and Tetraploid Grapes (Vitis vinifera and V. complex) and Recovery of Triploid Plants through Embryo Culture
- Degree of Abortion and Germination Rates in Triploid Seeds from Crosses between Diploid and Tetraploid Grapes (Vitis vinifera L. and V. complex)
- Estimation of the Degree of Self-incompatibility Reaction during Flower Bud Development and Production of Self-fertilized Seeds by Bud Pollination in Self-incompatible Citrus Cultivars
- Germinability of Embryos during Seed Development in Citrus (Rutaceae)
- Most Hypotetraploid Seedlings from Self-Pollinated Tetraploid Grapes ( Vitis complexes) Have Abnormal Cotyledons
- Morphological and Physiological Variation in Kakrol (Momordica dioica Roxb.)
- Variation of Glucosephosphate Isomerase and Phosphoglucomutase Isozymes in Vitis and Their use in Grape Breeding
- Hybridization and asymmetric introgression between Rhododendron eriocarpum and R. indicum on Yakushima Island, southwest Japan
- Seed Germination Response to Temperature and Salinity Stress in Lilim longiflorum and L. formosanum
- Production of Triploid Plants from Crosses between Diploid and Tetraploid Grapes (Vitis complex) through Immature Seed Culture and Subsequent Embryo Culture