汎用医薬品の好塩基球段階のアレルギー応答に対する影響

概要

論文の詳細を見る
An in vitro evaluation system for the commonly used medical drugs for allergic reaction was developed. Anti-DNP monoclonal IgE antibody sensitized cultured rat basophilic leucocyte cell line RBL-2H3 was stimulated by DNP-linked human serum albumin in the presence of various concentrations of the commonly used medical drugs, and the amounts of released histamine were estimated by HPLC. An antipyretic, aspirin and an anti-histamine, chlorpheniramine maleate were found to reduce the allergen-induced release of histamine ; and a β-blocker, propranolol and an αβ-stimulant, ephedrine hydrochloride were seen to enhance histamine release. Other drugs tested including NO-producing drugs, nitroglycerin and hydralazine, a Ca^<++> channel blocker, diltiazem, a loop diuretic, furosemide and a Ca^<++> drug, elcatonin, were found to have no effect on the allergen-induced release of histamine from basophilic leucocytes. Since these reactions could be explained by their molecular mode of actions, this system could be applied to the evaluation of the effects of general drugs on the first-step allergy reaction.
日本医療薬学会の論文
1996-10-10