The Acceleration Effect on Discoloration of New Coccine with Lysozyme in the Admixture of Alimezine Syrup, Inolin Syrup and Leftose Syrup
スポンサーリンク
概要
- 論文の詳細を見る
A study was made to find out how important the role of protein binding was in an admixture of syrups of A1imezine (Ali.) (containing new coccine as a coloring matter), Inolin (Ino.) and Leftose (Lef), which became discolored during the storage of 5.5 hr at room temperature. The discoloration of new coccine in the Ali. syrup from red (λ_<max> 510 nm) to yellow(λ_<max> 445 nm) was monitered at 510 nm using a photospectrometer. The acceleration effect on discoloration was found out with Ali. syrup, Ino. syrup and a substitute for Lef in which lysozyme chloride was replaced by bovine serum albumin (BSA), poly-L-lysine and chitosan. The discoloration was reduced with Ali. syrup, Ino. syrup, and water solution of poly-L-glutamic acid and L-lysine, a substitute for Lef. The discoloration of new coccine was not observed in the admixture of 0.01% new coccine, 0.1% Na_2SO_3 and 0.5% lysozyme chloride in the solution of the 0.01% sodium lauryl sulfate and 1 M sodium chloride. It is considered that the interaction of ionic binding between sulfonic group on the new coccine molecular and lysine residual group on the lysozyme chloride is an important factor for discoloration process. The reaction rate of discoloration in the syrup admixture is accelerated by protein binding of new coccine to the lysozyme chloride. The enzymatic activity of lysozyme chloride was not decreased with protein binding or discoloration of new coccine. The profile of electrophoresis of lysozyme chloride and BSA obtained after discoloration in the syrup admixture showed the same profile as that of standard samples.
- 日本医療薬学会の論文
- 1992-10-20
著者
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OHKUBO Tadashi
Department of Pharmacy, Hirosaki University Hospital
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SUGAWARA Kazunobu
Department of Pharmacy, Hirosaki University Hospital
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Ohkubo Tadashi
Department Of Pharmacy Hirosaki University Hospital
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Sugawara Kazunobu
Department Of Clinical Pharmacy Aomori University School Of Pharmacy
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SUGAWARA Kazunobu
Department of Chemical Pharmacology, Pharmaceutical Institute, Tohoku University
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