肺癌動注療法におけるシスプラチンの血中動態

概要

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A method of high-performance liquid chromatography was developed in order to study the pharmacokinetics of cisplatin (CDDP) administration by bronchial artery infusion (BAI). Following ultrafiltration of serum with centrisart I (mol. wt. cut off<l0,000), platinum chelation by N, N-diethyl-dithiocarbamate (DDTC), extraction with chloroform and separation on an octadecylsilane-bonded silica column (TSK gel ODS 120T, 4.6 I.D.×150mm), the eluted components were detected by UV monitoring at 254 nm. The mobile phase consisted of meth-anol-chloroform-water (68:3:29). Nickel chloride was used as an internal standard. There was a linear relationship between the peak height ratio and the concentration of CDDP in the serum in the range of 0.1 to 10μg/ml. The detection limit was 0.02μg/ml (S/N=3) in serum. The overall analytical recovery of CDDP was 78.4-92.2% in the concentration in the range of 0.1 to 10μg/ml. Serum concentrations of ultrafiltratable CDDP were determined by following 50mg/body and 50mg/m^2 BAI of CDDP to lung cancer patients, and the effects of the two doses were compared. After BAI, the mean concentration of ultrafiltratable CDDP showed that the drugs behaved according to the rules of the one-compartment open model and the respective halflives were 27.75min (50mg/body) and 21.88min (50mg/m^2), which were significantly different.
日本医療薬学会の論文
1988-08-20