高遠液体クロマトグラフィーによるキョウニン水中のマンデルニトリルの定量

概要

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Mandelonitrile in Apricot Kernel Water was studied by high-performance liquid chromatography using an octadecyl bonded stationary phase. According to the Kawai method, 0.5 ml of Apricot Kernel Water is treated with 0.3 ml of 1% hydroxylamine hydrochloride solution, which react with free benzaldehyde. After an excess of hydroxylamine is removed by adding 0.2 ml of acetone, the mixture is treated with 0.3 ml of 10% ammonia solution. And then, the aqueous solution is saturated with NaCl, and the benzaldehyde produced corresponding to mandelonitrile is extracted with 2 ml of ethl acetate containing 0.6 mg of α-(o-tolylazo)-β-naphthylamine as an internal standard. This method was applied to analysis of a colored preparation composed of Apricot Kernel Water, Senega Syrup and M.Verdau I (3:5:5) or Apricot Kernel Water, Liquid Brocin and M.Verdau I (3:5:10). With this method, the amount of benzaldehyde produced corresponding to mandelonitrile was determined with in 1.68% error over the range from 0.5-5.0 μ/ml.
日本医療薬学会の論文
1987-02-20