Expression of Corticotropin-Releasing Hormone Type 1 Receptor in Paraventricular Nucleus after Acute Stress
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概要
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We have previously proposed the existence of ultrashort loop-positive feedback regulation of corticotropin-releasing hormone (CRH) in the hypothalamus. To gain a better understanding of this effect, we performed double-label in situ hybridization to identify the neurons in the paraventricular nucleus (PVN) that express CRH type 1 receptor (CRH-R1) following stress. We also conducted immunohistochemistry to determine whether CRH-R1 mRNA was translated to CRH-R1 protein in the PVN. Thirty-minute restraint stress given to male Wistar rats increased c-fos mRNA expression primarily in the CRH-producing neurons of the parvocellular PVN. Small numbers of vasopressin and oxytoxin-producing cells were also labeled by c-fos probes. Approximately 70% of CRH-R1 positive neurons exhibited CRH mRNA 2 h after the beginning of stress, while only a small percentage of the vasopressin and oxytocin-producing cells coexpressed CRH-R1 mRNA. CRH-R1 immunoreactivity, which was detected in the perikarya and fibers of PVN neurons, appeared to increase in response to stress, though this was not statistically significant. Pretreatment with a selective CRH-R1 antagonist, CP-154,526,significantly attenuated stress-induced corticotropin (ACTH) secretion as well as c-fos mRNA expression in the PVN. These results demonstrate that acute stress increases neuronal activation and CRH-R1 mRNA expression primarily in CRH-producing neurons of the parvocellular PVN, that CRH-R1 message is translated to CRH-R1 protein, and that PVN neurons are activated at least in part through CRH-R1 under acute stress. The data further support the possibility of feedback regulation of CRH itself in CRH-producing neurons.
- 日本医科大学の論文
- 2002-03-25
著者
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Naruse Mitsuhide
Department Of Medicine Tokyo Women's Medical University
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Naruse Mitsuhide
Department Of Endocrinology Kyoto Medical Center
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Imaki Toshihiro
Department of Bioregulation, Institute of Gerontology, Nippon Medical School
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Katsumata Harumi
Department of Bioregulation, Institute of Gerontology, Nippon Medical School
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Miyata Mariko
Department of Physiology, Tokyo Women's Medical University
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Imaki Junko
Department of Anatomy, Nippon Medical School
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Minami Shiro
Department of Bioregulation, Institute of Gerontology, Nippon Medical School
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Imaki Junko
Department Of Anatomy Nippon Medical School
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Minami Shiro
Department Of Bioregulation Institute Of Development And Aging Sciences Nippon Medical School
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Miyata Mariko
Department Of Information Physiology National Institute For Physiological Sciences
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Miyata Mariko
Department Of Physiology Tokyo Women's Medical University:laboratory For Learning And Memory Br
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Imaki Toshihiro
Department Of Bioregulation Institute Of Development And Aging Sciences Nippon Medical School
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Katsumata Harumi
Department Of Bioregulation Institute Of Gerontology Nippon Medical School
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Naruse Mitsuhide
Department of Endocrinology and Metabolism, National Hospital Organization Kyoto Medical Center
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