三種類の基質で測定される土壌ホスフォジエステラーゼ活性のpH曲線および起源

概要

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Bis-(p-nitrophenyl) phosphate (BPNP), thymidine-3'-p-nitrophenyl phosphate (T3'PNP) and thymidine-5'-p-nitrophenyl phosphate (T5'PNP) were used as substrates for the measurement of phosphodiesterase activity in soils which were treated with antibiotics. Addition of chloramphenicol or cycloheximide to soils, which were oven-dried and then re-moistened and inoculated with the original soil of 2%, caused the selective inhibition of bacterial and fungal growth. The inhibition of bacteria increased but that of fungi decreased phosphodiesterase activities in a larch forest soil measured with the three kinds of the substrates. The activities were optimal at pH 4.6-5.6. BPNPase activity in soils where Trichoderma sp. was inoculated and then incubated, was also optimal at pH 5. These results indicated that fungi were more important sources of phosphodiesterases in this soil. In contrast, BPNPase and T3'PNPase activity. Activities of T3'PNPase and T5'PNPase showed a similar level in 5 soil samples, suggesting that soils hydrolyze either 3'- or 5'-phosphodiester bond from 3'-side or 5'-side of deoxyribonucleotides and ribonucleotides.
社団法人日本土壌肥料学会の論文
1990-02-05