ペーパークロマトグラフ及び瀘紙電気泳動法による Rye Diffusate の開花促進成分に関する定性試験

概要

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The flower- promoting activity of each fraction of rye diffusate was assayed to concentrate the study of flowering substances, adopting (tiny) seeds of annual Meadow grass, Poa annua as subjects. As far as the flowering of Poa annua was concerned, the mixture of the fractions expedited flowering best. It is interesting to notice in Fig. 1 that the flowering curve of Poa annua treated with the mixture seems to be a combination of two curves of those treated with native auxin and a nucleotide-like substance. To chase this unknown nucleotide-like substance, paper chromatography and paper electrophoresis were carried out in this experiment. According to the Rf values and electric migrations of rye diffusate, adenosine triphosphate, adenosine monophosphate, uridylic acid and fructose-6-phosphate, it is considered that the effective substance may be the one which has energy-rich phosphates like uridine diphosphate or uridine triphosphate. If one of those substances truly controls the flowering of winter crops, such as wheat or rye, devernalization caused by warm temperature may be related with the heat-labile property of the substance. It would be too early to say that the flowering hormone is a complex of a native auxin and UDP (or UTP)-like nucleotide. However, if the flowering hormone were not simple in composition, these effective substances investigated in this experiment may be the core of "Florigen".
日本作物学会の論文
1959-09-01