ADENOSINE DEAMINASE ACTIVITY IN SOILS

概要

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Adenosine deaminase activity six soil samples was measured. One g of moist soil was incubated with 0.02 adenosine in 0.1 M phosphate buffer (pH 7.6) and toluene in a shaking water bath (30℃) for 72 h. Inosine and NH^+_4 were detected in the filtrate as degradation products of adenosine; inosine was identified by paper chromatography and NH^+_4 was determined by Nessler's method. The adenosine deaminase activity in soils was completely inhibited by autoclaving (120℃, 15 min). The relation between substrate concentration and reaction rate followed the Mechaelis-Menten equation. The optimum pH for adenosine deaminase activity in soils ranged from about 7 to 8. The adenosine deaminase activity in soils ranged from about 1.51 to 4.33 munit (nmol per min) per g of dry soil at 30℃.
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