IMPROVEMENTS IN THE METHOD FOR FRACTIONAL DETERMINATION OF SOIL ORGANIC NITROGEN
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概要
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To reinvestigate the experimental conditions for determining various forms of organic nitrogen, known nitrogenous compounds were hydrolysed and steam distilled using Bremner's apparatus. The recoveries of amide-N, α-amino-N, and hexosamine-N were determined. More than 24 hr were required to complete the hydrolysis of protein and chitin with boiling 6 N HCl. Under these conditions, decomposition of glucosamine occurred and increased with heating time, resulting in an increase of the amide-N fraction. Little glucosamine was decomposed to ammonium-N by hydrolysis with 1 N HCl at 100℃. However, chitin was incompletely hydrolysed to glucosamine under these conditions. In contrast, amide-N was almost completely recovered. The amount of hexosamine-N was determined by subtracting the nitrogen distillable with MgO in the 1 N HCl hydrolysate from the nitrogen distillable with phosphate-borate buffer in the 6 N HCl hydrolysate. Based on these results, improved procedures for determining organic nitrogen are proposed. These procedures were evaluated by comparing the results for 20 soils with those obtained by Bremner's methods. The contents of hydrolysable ammonium-N in Thai soils were significantly higher than those of Japanese soils. According to Bremner, it is difficult to eatablish the origin of such accumulated nitrogen. However, the hexosamine-N contents of Thai soils obtained by the improved procedure were significantly higher than those of Japanese soils. This is probably related to amino sugar compounds accumulated within the microbial residues in tropical soils. These results suggest that the improved method represents a promising procedure for characterizing soil organic nitrogen especially in the case of tropical soils.
- 社団法人日本土壌肥料学会の論文
著者
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Yonebayashi Koyo
Faculty of Agriculture, Kyoto Prefectural Unirersity
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Hattori Tomoo
Faculty of Agriculture, Kyoto Prefectural University
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Hattori Tomoo
Faculty Of Agriculture Kyoto Prefectural University
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Yonebayashi Koyo
Faculty Of Agriculture Kyoto Prefectural University
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