Serological Properties and Intrinsic Antibiotic Resistance of Soybean Bradyrhizobia Isolated from Thailand
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概要
- 論文の詳細を見る
A group of Bradyrhizobium strains isolated from soybean plants in Thailand did not correspond to any known DNA homology groups of Bradyrhizobium japonieum and Bradyrhizobium elkanii reported by Hollis et al. (J. Gen. Microbiol., 123, 215-222, 1981). To clarify the phenotypic characteristics of the group, serological properties and intrinsic antibiotic resistance (IAR) profile of 94 Thai strains were compared with those of USDA and Japanese strains. Indirect ELISA tests for each Thai strain were performed against polyclonal antisera prepared against 15 USDA standard serotype strains of B. japonicum and B. elkanii. Among the 94 Thai strains tested, 36 which were previously identified as B. elkanii, with the exception of one strain, were strongly responsive to an antiserum prepared against USDA 31. The remaining 58 strains, with the exception of two strains, showed multiple cross reactions which were peculiar to the Thai strains. These serological reaction patterns did not correspond to any known serogroups labeled as B. japonicum and B. elkanii. In the IAR test, the taxonomically unknown Thai soybean bradyrhizobia exhibited a high level of resistance to neomycin (50 μg/mL), polymyxin (50 μg/mL), nalidixic acid (15 μg/mL), and kanamycin (15 μg/mL). Kanamycin could thus be useful in combination with neomycine and nalidixie acid for distinguishing between the unknown Thai strains and strains of B. japonicum anti B. elkanii. Our results demonstrated that the unknown Thai strains were serologically and IAR-phenotypically remote from both B. japonicum and B. elkanii.
- 社団法人日本土壌肥料学会の論文
著者
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Tsuchiya Kenichi
National Institute Of Agrobiological Resources
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Ando S
National Inst. Livestock And Grassland Sci. Nishinasuno Jpn
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YOKOYAMA Tadashi
National Institute of Agro-Biological Sciences
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Yokoyama Tadashi
National Institute Of Agrobiological Resources:(present) Tokyo University Of Agriculture And Technol
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Tsuchiya Kenichi
National Inst. Agrobiological Resources Tsukuba Jpn
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Ando Shotaro
Japan International Research Center for Agricultural Sciences
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Tsuchiya Kenichi
National Agricultural Research Center for Western Region
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