Confocal laser scanning microscopic observation of the pattern of tetracycline labeling in the incisors of rats with a low calcium diet
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概要
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In order to determine how Ca affects the growth of rat incisors, three-week old male rats were divided into four groups and fed controlled diets for three weeks. Group 1 was fed a low calcium diet for 13 days followed by a standard diet; group 2 was fed a standard diet for 13 days followed by a low calcium diet; group 3 was fed a low calcium diet only ; and the control group was fed a standard diet only. Dentin growth was examined using the tetracycline (TC) labeling method. A confocal laser scanning microscope (CLSM) was employed to examine the pattern of TC labeling, to measure the thickness and area of the layers between each two labeling lines, and to measure the cross-sectional areas of the incisors and dental pulps. In addition, body weight and serum Ca and serum P levels were measured to investigate their relationship to the amount of mineralized tissue growth in the dentin. After the diet changed, a marked increase in body weight was observed in group 1 and a gradual increase in the rats of group 2. Compared with the controls, the increase in group 3 was slow and gradual. The observed values for serum Ca in group 3 were significantly lower than in the controls. Group 3 had the highest values for cross-sectional area of the dental pulp. No significant differences were observed for the cross-sectional area of incisors among the four groups. Furthermore, group 1 showed a prominent increase in both thickness and area of mineralized tissue, after alteration of the diet. A slow and gradual reduction in both thickness and area were observed in group 2 after changing the diet. The thickness and area of mineralized tissue growth observed in group 3 were significantly lower than in the controls. These results suggest that the amount of Ca ingested has a profound effect on dentin growth in rats.
- 大阪歯科学会の論文
著者
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ITO Shigeki
Department of Fixed Prosthodontics, School of Dentistry, Iwate Medical University
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Ito Shigeki
Department Of Penodontology Matsumoto Dental University
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Ito Shigeki
Department Of Applied Chemistry Nagoya Institute Of Technology
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