河川水中の変異原物質の検索 : シンポジウム : I. 環境汚染変異原

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Water samples from the Nishitakase River in Kyoto, especially taken at sites below sewage j plants, show significantly high mutagenicity in the Ames test. Two mutagenic compounds (I and II) were isolated by Sephadex LH-20 column chromatography and HPLC on ODS column from material adsorbed to 27 kg of blue cotton. Based on the UV, mass and ^1H-NMR spectra of both (I) and (II) , and X-ray crystallography data of a debrominated derivative of (I) , the structures of (I) and (II) were determined to be 2- [2- (acetylamino)-4- [bis (2-methoxyethyl) amino] -5-methoxyphenyl] -5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-1) and 2- [2- (acetylamino) -4-IN- (2-cyanoethyl) ethylamino] -5-methoxyphenyl] -5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-2) , respectively. PBTA-1 and -2 were synthesized from azo dyes that had the correspond-ing substitutional groups by reduction and chlorination. Since all of the spectra of PBTA-1 and -2 coincided with those of (I) and (II) , (I) and (II) were concluded to be PBTA-1 and -2, respectively. PBTA-1 and -2 were newly identified potent mutagens, which induced 3.000,000 and 3,200,000 revertants of Salmonella typhimurium YG1024 per microgram, respectively, in the presence of S9 mix. They may be produced from azo dyes during industrial processes in dyeing factories and treatment at sewage plants. (This paper, in a session chaired by Teruhisa Hirayama and Keiji Wakabayashi, was presented to the symposium "Mutagenicity of environmental pollutants", organized by Teruhisa Hirayama, at the 27^<th> annual meeting of the Environmental Mutagen Society of Japan, and held at the Mielparque Osaka in Osaka, Japan, November, 24-26, 1998.)
日本環境変異原学会の論文
1999-07-12

河川水中の変異原物質の検索 : シンポジウム : I. 環境汚染変異原

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