Muta^<TM> Mouse lacZ導入遺伝子の塩基配列

概要

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The nucleotide sequence of the lacZ transgene of the lacZ transgenic mouse strain (Muta^<TM> Mouse, 40.6) has been determined. The lacZ transgene has 15 base-pair (bp) of an insertion of 5'-AA TTC CCG GGG ATC C-3' between the 25th and 26th nucleotides of the complete original E. coli lacZ gene demonstrated on the Big Blue Website of the internet, which is based on the report of Kalnins et al. (1983) , resulting in 3087 bp. The lacZ transgene also carries mutations of three base-pair substitutions of A to G at the 312th, T to C at the 573rd, and C to A at the 3024th bp of the original E. coli lacZ gene. The nucleotide number of the transgene determined in this study is inconsistent with the 3126 bp and 3096 bp as reported by Gossen et al. (1989) , and by Vijg and Douglas (1996) , respectively.
日本環境変異原学会の論文
1999-02-28