塩素処理した下水細菌培養液中に生成する変異原物質

概要

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When sewage bacteria were grown with glucose as a sole source of carbon and energy, precursors of direct mutagenic substances were produced in the culture solution. The culture solution showed no significant mutagenic activity by itself. However after chlorination, it exhibited dose-related mutagenic activity in Salmonella typhimurium TA100 without S9 mix. About 80 % of the mutagenic precursors were recovered in the precipitate fraction obtained by centrifugation for 20 min at 12000×g. The mutagenic substances in the chlorinated culture solution were adsorbed to a solid phase extraction column (Excelpak SPE-GLF cartridge) and were effectively eluted with ethyl acetate. We could detect the formation of glyoxal and methylglyoxal in the chlorinated culture solution, but the amounts of these aldehydes were too small to consider as major mutagens. Further studies are required to identify the major mutagens.
日本環境変異原学会の論文
1999-02-28