微生物変異原性試験における各種溶媒の適合性の検討

概要

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We examined six different solvents to determine their compatibility with the Salmonella and Escherichia/microsome mutagenicity(Ames)assay. These solvents were acetone, 95% ethanol, ethylene glycol, glycerol formal, formamide and 10% PLURONIC F-68 solution. The standard mutagens used were 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide, benzo(a)pyrene, 2-aminoanthracene, 9-aminoacridine hydrochloride monohydrate, sodium azide and bleomycin hydrochloride. Tests were carried out in five histidine-requiring strains TA98, TA100, TA102, TA1535 and TA1537 of S.typhimurium, and three tryptophan-requiring strains WP2 uvrA, WP2 uvrA/pKM 101 and WP2/pKM 101 of E.coli, both in the absence of and in the presence of metabolic activation(S 9 mix), in two separate experiments. All treatments were performed after a 20-min.pre-incubation procedure.50 μl of each test article solution and solvent was administered per plate. Five solvents(acetone, 95% ethanol, ethylene glycol, formamide and 10% PLURONIC F-68 solution)were found to be compatible with the eight test strains. Glycerol formal was found to be toxic(inhibiting growth)to TA102, TA1535 and TA1537. It is suggested that glycerol formal should not be used as a solvent in the Ames test.
日本環境変異原学会の論文
1998-04-10