げっ歯類の培養細胞と個体を用いる小核試験法の検討

概要

論文の詳細を見る
The cytokinesis-block method using cytochalasin B that was developed by Fenech and Morley for micronucleus assay using human lymphocytes was applied to the rodent CHL cell line. Consistent dose-response relationships were obtained in cultures treated with clastogens and an aneugen. Comparison with the chromosome aberration frequencies indicated that approximately 30 % of acentric chromosomes are expressed as micronuclei in the cells treated with mitomycin C and caffeine. The size distribution of the micronuclei suggested that the base-line frequency of micronuclei is mainly a reflection of mitotic dysfunctions rather than chromosome structural aberrations. Conditions for route and frequency of administration in the mouse micronucleus test were evaluated. Both intraperitoneal injection and oral gavage were acceptable as routes of administration in this test. Double dosing, with a 24 h interval and sampling 24 h after the second dosing, was the most sensitive protocol compared to single, triple, or quadruple dosing protocols. The micronucleus test using bone marrow and peripheral blood from exposed rats was evaluated. It was concluded that the rat micronucleus test, using either bone marrow or peripheral blood, can be used as an alternative to the mouse micronucleus test.
日本環境変異原学会の論文
2002-09-30