トリクロール醋酸による多角体蛋白のアルコールへの溶解
スポンサーリンク
概要
- 論文の詳細を見る
In the course of the procedure to determine nucleic acids in the polyhedral bodies, or the inclusion bodies of virus particles, of silkworms, it was observed that the major part of the bodies treated with trichloroacetic acid (TCA) immediately dissolved in alcohol. The material dissolved in alcohol was proved to be protein in the previous paper. By the presence of TCA, the polyhedral protein dissolves in acetone and in methyl alcohol as well as in ethyl alcohol, but does not dissolve in water, ether, and benzene. This phenomenon can be attributed to the specialities of both the protein and the acid. Only the polyhedral protein dissolved in alcohol containing TCA, and no protein having such a property was observed (Table 2). The dissolution of the protein in alcohol is caused by a minute amount of some special acids (Table 3). TCA has the strongest effect. The acids which have stronger effect are generally stronger acids, and hydrogen ion is an indispensable factor to dissolve the protein in alcohol (Table 4). However, the effectiveness of acids has no direct relationship to their dissociation constants, and the kind of anions seems to be more important. It is very interesting to note that the two most effective acids, TCA and perchloric acid, are similar to each other in their structures on the electronic aspects. They have likewise three polar radicals around an atom. A linear relationship is observed between the concentration of TCA and the solubility of the polyhedral protein (Fig. 1). The protein dissolves in alcohol by 1 g. of its nitrogen per 0.01 mole of TCA. The polyhedral protein treated with TCA became insoluble in alcohol by dialysis against distilled water in the refrigerator. But the dialysed protein dissolved in alcohol by a new addition of TCA. From the freshly prepared alcoholic solution which was saturated with the protein upon the definite amount of TCA, the latter was rapidly removed away and gel-formation was brought about by dialysis against alcohol in the refrigerator (Table 5). A hypothesis to explain these phenomena was proposed.多角体蛋白は微量の特殊な酸を含有する無水アルコールに溶解する. この現象は蛋白質及び酸の特異性に帰せられる. エタノールの外メタノ一ル, アセトンが溶媒として有効である. 有効な酸はある程度以上の強酸であるが,一般に酸の強さと該効果の間には一定の関係は認められない.この酸効果はトリクロール醋酸(TCA)が最も強い. TCAと溶解蛋白量の間には直線的関係がある.多角体蛋白のアルコール溶液から, TCAが透析によつて除去された. この様な多角体蛋白の溶解機作について考察を加えた.
- 九州大学の論文
九州大学 | 論文
- Sprague-Dawleyラットの脂質代謝,免疫機能に及ぼすイチョウ葉および竹エキス粉末の摂食効果
- 斜面, ライパリアンゾーンの水分状態と流域スケールの流出の関係 : 御手洗水試験流域における観測例
- SOI基板を用いた新規AWGデバイスの作製
- Si基板上へのSiC超薄膜形成とSiCをバリアー層とする共鳴トンネルデバイスの試作
- 「『葉隠』にみる家臣の『譜代』意識と御家の『家風』」