Purification and cDNA cloning of an insect defensin from larvae of the longicorn beetle, Acalolepta luxuriosa

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概要

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• We purified an antibacterial peptide from the larval hemolymph of a coleopteran beetle, Acalolepta luxuriosa. Using structure analysis and cDNA cloning, this peptide was identified as a novel member of the insect defensin family and named A. luxuriosa defensin 1. A. luxuriosa defensin 1 shares high sequence similarity with other defensins, especially coleopteran defensins. A dendrogram of coleopteran insect defensins based on sequence homology revealed that A. luxuriosa defensin is closer to Tenebrionoidea defensin than to Scarabaeoidea defensin, which parallels the evolutionary relationship of these coleopteran insects. Although A. luxuriosa defensin 1 was most homologous with Tenebrio molitor tenecin 1, its antibacterial spectrum was broader, affecting the growth of Gram-positive and Gramnegative bacteria, suggesting that the variability of the antibacterial spectrum results from small sequence differences.

• 2005-05-25

著者

• SATO Ryoichi

  Graduate School of Bio-Application and Systems Engineering, Tokyo University of Agriculture and Tech

• IMAMURA Morikazu

  Prion Disease Research Center, National Institute of Animal Health

• Imamura Morikazu

  Prion Disease Research Center National Institute Of Animal Health

• UEDA Kenjiro

  Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec

• SAITO Ayaka

  Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec

• Saito Ayaka

  Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech

• Ueda Kenjiro

  Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech

• Sato Ryoichi

  Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech

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