Unusual Proteolytic Processing of a δ-Endotoxin from Bacillus thuringiensis Strain Buibui by Larval Midgut-Juice of Anomala cuprea HOPE (Coleoptera: Scarabaeidae)

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概要

• 論文の詳細を見る

• The colepteran-active δ-endotoxin of Bacillus thuringiensis serovar japonensis strain Buibui had a so-called proteinase-resistant region of 50 kDa which is resistant to trypsin and larval midgut-juice of Bombyx mori. However, the δ-endotoxin of strain Buibui was degraded to smaller polypeptides of less than 20 kDa by larval midgut-juice of Anomala cuprea. In addition, the CryIAa δ-endotoxin was also degraded to smaller polypeptides by the midgut-juice. On the other hand, a mixture of trypsin and autoclaved A. cuprea midgut-juice degraded the δ-endotoxin to smaller polypeptides but the autoclaved midgut-juice with Staphylococcus aureus V8 proteinase or lysyl endopeptidase did not. These results suggest that A. cuprea midgut-juice may have a high activity to digest the δ-endotoxins, for which a trypsin-like proteinase may play an important role. Moreover, A. cuprea ;midgut-juice may contain a factor(s) which cooperates with trypsin to digest δ-endotoxin into smaller polypeptides.

• 日本応用動物昆虫学会の論文
• 1997-11-25

著者

• SATO Ryoichi

  Graduate School of Bio-Application and Systems Engineering, Tokyo University of Agriculture and Tech

• Iwahana H

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Iwahana Hidenori

  Department Of App Lied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• SUGIMURA Masahiro

  Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an

• Sugimura M

  National Inst. Agrobiological Sci.

• Sato Ryoichi

  Graduate School Of Bio-applications & Systems Engineering Tokyo University Of Agriculture And Te

関連論文

• Localization of a novel 252-kDa plasma membrane protein that binds Cry1A toxins in the midgut epithelia of Bombyx mori
• Bacillus thuringiensis Cry Toxins Bound Specifically to Various Proteins via Domain III, Which Had a Galactose-Binding Domain-Like Fold
• cDNA and deduced amino acid sequence of apolipophorin-III from Agrius convolvuli (Sphingidae: Lepidoptera)
• Purification and cDNA cloning of an insect defensin from larvae of the longicorn beetle, Acalolepta luxuriosa
• Identification of a New Pheromone-Binding Protein in the Antennae of a Geometrid Species and Preparation of Its Antibody to Analyze the Antennal Proteins of Moths Secreting Type II Sex Pheromone Components
• Bacillus thuringiensis Cry Toxins Bound Specifically to Various Proteins via Domain III, Which Had a Galactose-Binding Domain-Like Fold
• GTD Analysis for Evanescent Modal Excitation
• Deletion Mutants of the Gene Encoding δ-Endotoxin Specific to Scarabaeid Beetles : Minimum Region of the Gene Required to Express the Activity
• Comparative Studies of Growth Characteristic and Competitive Ability in Bacillus thuringiensis and Bacillus cereus in Soil
• Factors Affecting Susceptibility of Aedes albopictus SKUSE (Diptera: Culicidae) Larvae to Smittium morbosum SWEENEY (Trichomycetes: Harpellales)
• Unusual Proteolytic Processing of a δ-Endotoxin from Bacillus thuringiensis Strain Buibui by Larval Midgut-Juice of Anomala cuprea HOPE (Coleoptera: Scarabaeidae)
• Extra-and intracellular signaling pathways regulating nodule formation by hemocytes of the silkworm, Bombyx mori (Lepidoptera : Bombicidae)

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