成長期ラットにおける骨中のカルシウム、リンの測定 : 蛍光X線装置を用いて

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We know well the contents of minerals of the growing rat bones were changed quantitatively by many factors. The object of our experiment how effect does the difference hardness diet have the changes in the contents of calcium and phosphorus in the bone. In this study we used 4-weeks-old female rats which were fed the normal diet on a continuing basis throughout the experiment. The mandible and femur were extracted at 4-weeks-old, 6-weeks-old and 8-weeks-old. The bones were fixed in a 10% formalin solution, washed with distilled water, and air-dried. Bone contents determined sites: The head of the mandible (Mandible I), the region which includes teeth anterior to a line perpendicular to the tangential line drawn at the inferior margin of mandible (Mandible II) and the head of the femur (Femur). The sample bones were pulverized and the result powder was shaped in ring under a pressure. The contents of calcium and phosphorus of samples were then analyzed quantitatively by a X-ray fluorescent analyzer (ZSX101e Rigaku Industry). The results: Mandible I; The contents of calcium and phosphorus did not increase with growth. Mandible II and Femur; The contents of them tended to increase with growth. ZSX101e is widely used for quantitative and quantitative analysis of food and mineral contents. The results of the present study showed similar tendencies to those of Akiyama et al.; who employed a traditional and widely used DXA method. On the basis of our findings, it was considered that the analyzer is useful for quantitatively determining bone content. Mandible I did not show any increases as the rats aged, likely because growth of the mandible head begins later than 8-weeks-old in these rats. In the future, we would like to examine the changes in contents in bone over the passage of time, using a later number of subjects and longer aging periods.
硬組織再生生物学会の論文

成長期ラットにおける骨中のカルシウム、リンの測定 : 蛍光X線装置を用いて

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