赤-近赤外測定用分光蛍光光度計の光量子計色素の検討

概要

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Spectrofluorophotometric quantum counter dye for fluorescent samples under excitation of light 600-700 nm wavelength range was investigated. Five kinds of cyanine, three of methylene blue and three of phthalocyanine dyes respectively, were provided for examination. As the results of characteristic measurements, we found that methylene blue trihydrate has most superior characteristics for absorption-fluorescent spectra and for stabirity. Measuring the fluorescent spectra of fluorescent samples (cyanine dye) under various exciting light intensities, using the methylene blue trihydrate for spectrofluorophotometric quantum counter dye, the practical accuracy of this quantum counter dye was proofed. Adjustment of the compensator of spectrofluorophotometer enabled us to measure corrected fluorescent spectrum until wavelength by 830 nm. Consequently, relative fluorescent quantum yields for samples such as cyanine dyes has become obtainable till nearinfrared wavelength range.
東京工芸大学の論文