二種類の光量子計色素を用いた広帯域分光蛍光光度計の製作

概要

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Wide wavelength range spectrofluorophotometer, exchangeable for short (300〜600 nm) and for long (485〜825 nm) wavelength compensating channels, has been designed on the base of Shimazu RF-502 spectrofluorophotometer, using Rhodamine B and Methylene blue as quantum counter dyes. Making of compensator suitable for this system. and spectral energy correction on fluorescent spectrometer and on photomultipliyer by standard tungsten lamp have been examined. Moreover to connect personal computer with this wide wavelength range spectrofluorophotometer an input preamplifier and programs for data treatment were designed, and consequently real time operation become enabled. 0n the results of above experiments the wide wavelength spectrofluorophotometric measurements with highly accuracy has becom expected, comparing fluorescent samples having long wavelength spectrum such as cyanine dyes with that of short wave characteristics having well known fluorescent quantum yield.
東京工芸大学の論文
1986-01-15