カイコ胚子形成過程におけるタンパク質合成の変動

概要

論文の詳細を見る
Protein synthesis in developing embryos of Bombyx mori was studied by means of microinjection experimens using tritium-labeled precursors and actinomycin D(AD). The incorporation of ^3H-leucine into the acid-insoluble fraction was shown to be most active on the first day (stages 3-4) and during the fifth to ninth days (stages 6-20) after oviposition. In the diapausing egg the incorporation of ^3H-leucine during stages 3 to 4 was not affected by AD, while the incorporation at the later stages was appreciably inhibited by AD. Two-dimensional gel electrophoresis followed by autoradiography using ^<35>S-methionine demonstrated the existence of stage-specific peptides which were synthesized at different phases of development. The present results suggest that the translational event in the early stages of embryogenesis depends on pre-existing maternal mRNAs, and at the later stages of the embryonic development it depends upon newly synthesized mRNAs.
日本応用動物昆虫学会の論文
1984-05-25