The nature and titer of juvenile hormone in the brown planthopper, Nilaparvata lugens (Homoptera: Delphacidae) in relation to wing morphogenesis and oocyte development
スポンサーリンク
概要
- 論文の詳細を見る
Whole body extracts from nymphs and adults of the brown planthopper, Nilaparvata lugens were analyzed for the known insect juvenile hormones (JHs) by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring. JH was separated successively by solvent extraction, chromatography on an aluminum oxide column and high performance liquid chromatography. JH III was identified as the only type of JH in both stages of this species. As the levels were very low for quantitative determination of JH III by GC-MS, we prepared haemolymph juvenile hormone binding protein (JHBP) of Locusta migratoria which had a high affinity for JH III and used it in a competitive binding assay to quantify JH III in the haemolymph of the brachypterous and macropterous pure lines of N. lugens. However, the JH III titer developmental profiles were similar in the brachypterous and macropterous fifth (final) stadium nymphs. JH III titers in the haemolymph of the brachypters were significantly higher than those of macropters, coinciding with earlier development of oocytes in the brachypters, which supported the regulation of ovarian development by JH III.
- 日本応用動物昆虫学会の論文
- 2002-02-25
著者
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Bertuso Arlene
Laboratory Of Applied Entomology Department Of Applied Biological Sciences Faculty Of Agriculture Sa
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Tojo Sumio
Laboratory of Applied Entomology, Department of Applied Biological Sciences, Faculty of Agriculture,
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Tojo Sumio
Laboratory Of Applied Entomology Department Of Applied Biological Sciences Faculty Of Agriculture Sa
関連論文
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