Phagocytic Activities of Haemocytes Separated by Two Simple Methods from Larvae of Two Lepidopteran Species, Agrotis segetum and Galleria mellonella
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概要
- 論文の詳細を見る
Differential cell fractionation (D.F.) and nylon wool column (N.W.) methods, both utilizing different calcium ion requirements among immunocytes for adhesion to non-self, were adopted to prepare immunocyte monolayers from Agrotis segetum and Galleria mellonella larvae. Monolayers prepared by the D.F. method were occupied predominantly by granulocytes, and those prepared by both D.F. and N.W. methods showed high purities of plasmatocytes (PL) and granular plasmatocytes (GPL). Granulocytes separated by the D.F. method in vitro showed high phagocytic activities against Indian ink particles and FITC-labeled silica beads, while PL and GPL prepared by both methods actively engulfed only FITC-labeled silica beads, similarly to granulocytes, but they could not phagocytose the Indian ink particles. Essentially similar results were obtained with monolayers from the two lepidopteran insects.
- 日本応用動物昆虫学会の論文
- 1995-05-25
著者
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Tojo Sumio
Laboratory Of Applied Entomology Department Of Applied Biological Sciences Faculty Of Agriculture Sa
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Tojo Sumio
Laboratory Of Entomology Department Of Applied Biological Sciences Faculty Of Agriculture Saga Unive
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YOKOO Shinya
Laboratory of Entomology Department of Applied Biological Sciences, Faculty of Agriculture, Saga Uni
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GOTZ Peter
Institut fur Zoologie Freie Universitat Berlin
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Yokoo Shinya
Laboratory Of Entomology Department Of Applied Biological Sciences Faculty Of Agriculture Saga Unive
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