Improvement of Infectivity of Metarhizium anisopliae(METSCHNIKOFF)to Anomala cuprea HOPE(Coleoptera:Scarabaeidae)by Ultraviolet Irradiation of Protoplasts

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概要

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• Enhancement of infectivity of a wild strain of Metarhizium anisopliae, F1011, against Anomala cuprea was attempted by Ultraviolet irradiation of the protoplasts isolated from mycelia. The protoplasts were regenerated in L-broth containing 2% agar in 0.7 M KCl. Among 38 regenerants obtained, the regenerant No.31 (RNO31), which originated from the protoplasts irradiated by UV for 30 s, was 21 times more virulent than the original strain F1011 against 1st instar larvae of A. cuprea, based on LC_<50> values. Fewer days were required to achieve a 50% death rate by RNO31 than by F1011. RNO31 also exhibited stronger insecticidal activity against Popillia japonica and Anomala daimiana than did F1011. The number of surviving larvae of A. cuprea in the RNO31 treated pot was lower than that in the F1011 treated pot. Effects of temperatures and agricultural chemicals on the growth of RNO31 were similar to those for the F1011, whereas the effect of pH values was not. Germination rate of conidia from RNO31 was about 2.5 times higher than that from F1011 at 16 h after the inoculation.

• 日本応用動物昆虫学会の論文
• 1993-11-25

著者

• Fujiie Azusa

  Chiba Prefectural Agicultural Experiment Station

• YOKOYAMA Tomoko

  Chiba Prefectural Agriculture Research Center

• FUJIKATA Masahiro

  Faculty of Horticulture, Chiba University

• Yokoyama Tomoko

  Chiba Prefectural Agricultural Experiment Station

• Fujikata Masahiro

  Faculty Of Horticulture Chiba University:the Consumers'co-operative Society Co-op Kanagowa

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