A New Fixation Method for Preparing Mite Specimens for Optical and SEM Microscopic Observations
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概要
- 論文の詳細を見る
A new fixation method for preparing mite specimens was developed. The dilutions of 99.5% methanol and 99.3% acetic acid (hereafter called as MA fluids) were useful for the fixation of cuticular epidermis and stretching the legs of tetranychid and phytoseiid mites. Comparisons between the MA fluids and the fixatives heretofore in use revealed that the 70% ethanol fluid, which had been usually adopted for mite fixative, was not good for making mite exo-skeletonic specimens. After fixation with the MA fluids, mites were maintained in good condition in 70% ethanol. there was no bad effect of the MA fixative on the length of leg I of mites mounted in HOYER's medium one week after the fixation. These fixatives were also useful as for the preliminary preparation of the mites for scanning electron microscopy.
- 日本応用動物昆虫学会の論文
- 1992-08-25
著者
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SAITO Yutaka
Laboratory of Animal Ecology, Graduate School of Agriculture, Hokkaido University
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OSAKABE Masahiro
Akitsu Branch, Fruit Tree Research Station
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Osakabe Masahiro
Akitsu Branch Fruit-tree Research Station
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Osakabe Masahiro
Akitsu Branch Fruit Tree Research Statian
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Saito Yutaka
Laboratory of Animal Ecology Graduate School of Agriculture, Hokkaido University
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