Mode of Action of Bacillus thuringiensis δ-Enodotoxn : Relative Roles of Spores and Crystals in Toxicity to Pieris, Lymantria and Ephestia Larvae

概要

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The relative roles of spores and crystals of Bacillus thuringiensis in toxicity to Pieris rapae (fourth instars), Lymantria dispar (fourth and sixth instars) and Ephestia cautella (fourth instars) were studied using absolutely pure spores and crystals.Spore-free pure crystals and its extract alone showed high toxicity even to Ephestia which has very low susceptibitity to this bacterium. Although spores were far less toxic than crystals, the presence of spores enhanced the toxicity of crystals more than twofold. Mixtures of B. thuringiensis crystals and B subtilis spores produced the same mortality as crystals alone, although B. subtilis spores as well as B. thuringiensis spores germinated and subsequently proliferated vigorously in the gut. Under the presence of antibiotics, no lethal effect of B. thuringiensis spores was observed. It is concluded that the presence of spores is not essential in the killing of any insects by δ-endotoxin. No systemic infection was found in diseased living larvae following any of our experimental treatments.
日本応用動物昆虫学会の論文
1980-11-25