Further Studies on the Dispersion-Inducing Substances of the German Cockroach, Blattella germanica (LINNE)(Blattaria : Blattellidae)

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Some biological and chemical nature of the dispersion-inducing substances of Blattella germanica was studied by preference tests for the first instar nymphs of the same species and by gel filtration chromatographies.As the results, either the saliva collected from the mouth parts and the salivary reservoir or the crop content and its turbid counterflow from the mouth parts showed repellency.The sex, stage, and species specificities of the substances were lax when examined with the saliva of B. germanica, Periplaneta americana, and P. fuliginosa.The dispersion-inducing substances were soluble in water and heat stable but not extractable with organic solvent. The substances were partially purified on Sephadex G-25 and G-100 gel filtration column chromatographies; they were cluted into at least two different molecular weight fractions. Their molecular weights were estimated to be about 70,000-100,000 and less than 5,000; they were supposed to have proteinaceous nature.
1984-05-25