LATEX AGGLUTINATION TEST : A SIMPLE, RAPID AND PRACTICAL METHOD FOR BOVINE SERUM CRP DETERMINATION

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概要

• 論文の詳細を見る

• A semi-quantitative latex agglutination test for bovine serum CRP levels has been established by mixing diluted serum (or diluted standard serum) with a 1% latex suspension containing 0.489 μm latex particles coated with affinity-purified antibody at a ratio of 20 μg/mg latex. The agglutination was performed on a glass slide in a moist chamber at room temperature with 45 min. incubation. This test is reliable, reproducible and the results correlate with those of the single radial immunodiffusion (SRID) test. The effect of low temperature storage on CRP concentration revealed a 30% degradation of CRP during 2 years storage at 4℃. The possible role of EDTA addition to prevent a decrease in serum CRP concentration by freezing and thawing is also discussed.

• 北海道大学の論文
• 1992-05-29

著者

• Saito M.

  Department of Engineering, Faculty of Engineering, University of Tokyo

• Syuto B.

  Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University

• Morimatsu M

  Hokkaido Univ. Sapporo Jpn

• SARIKAPUTI M.

  Department of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University

• MORIMATSU M.

  Department of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University

• YAMAMOTO S.

  Azabu College of Veterinary Medicine

• NAIKI M.

  Department of Veterinary Science, National Institute of Health

• Sarikaputi M.

  Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University

• Naiki M.

  Department Of Veterinary Science National Institute Of Health:to Whom Correspondence Should Be Addre

• Saito M.

  Department of Chemistry, College of General Education, Tohoku University

• Saito M.

  Department of Cardiology, National Cardiovascular Center

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