プロタミンの末端カルポキシル誘導体に関する研究 : I.エステル化法による分子量の決定

概要

論文の詳細を見る
Methylesters of clupeine and salmine were prepared from purified sulfates and crude picrates of the protamines (obtained from Clupea pallasii and Oncorhynchus keta, respectively) by two methods, in which esterification was carried out (A) by allowing them to stand at room temperature for prolonged periods of time in methanol containing 0.1N hydrochloric acid, or (B) by short time treatment under cooling with methanol saturated with hydrogen chloride. Methoxyl contents of the purified ester sulfates of both protamines were found to be 0.40〜0.41 per cent in the products obtained by method A when esterified thoroughly, and 0.39〜0.46 per cent by method B. Since these methoxyl groups are considered to be introduced only into C-terminal carboxyl groups of the protamine molecules, molecular weight of 6, 000〜6,200 (or 5,400〜6,400 by method B) was calculated for both protamines from the result. The molecular weight found in the above is also supported by the value (4,700〜5, 000) calculated from the result which was obtained by absorption measurement of the DNP group introduced to the free amino or imino group (N-terminal group) of the protamine methylesters.
宇宙航空研究開発機構の論文
1958-03-13