ミドリシャミセンガイの脂質についての研究(第1報) : 単純脂質について

概要

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The composition of fatty acids and sterols obtained from the simple lipids fraction of Lingula unguis was studied. (1) The simple lipids were extracted with acetone from the tissues of L. unguis. The extract was washed by the Folch's partition method and then with acetone. (2) Acetone soluble lipids(simple lipids) were fractionated on silicic acid column chromatography. The main fractions were triglycerides (27.5%), free fatty acids (17.4%) and free sterols (10.6%). (3) Triglycerides were subjected to methanolysis with 10% methanolic HC1, and the resulting fatty acid methyl esters were determined by gas chromatography (GC). The main fatty acids were C_<16:0> (22.1%), C_<16:1> (10.3%), C_<20:5> (22.2%), and C_<20:6> (13.3%). (4) Sterols were isolated from unsaponifiable fraction by preparative thin layer chromatography. Sterol composition was determined by GC and GC-Mass spectrometry as trimethylsilyl derivatives. The main sterols identified were cholesterol, cholestanol, brassicasterol, 24-methylcholesterol and 24-methylenecholesterol.
1978-12-01