高性能薄層クロマトグラフィーによる食品中の着色料の分離に関する研究(第II報) : DEAE-Sephadexを用いる分離・定性法

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The dyes in foods were separated and purified by DEAE-Sephadex ion exchange chromatography, and the separated dyes were analysed by high performance thin-layer chromatography. 1) The adsorption of tested dyes to DEAE-Sephadex was made at pH 2.8〜11.7 of the extract solutions. 2) The quantity of dyes adsorbed to 0.5 mI Sephadex suspension was maximum in Indigo Carmine, amounting to 90μg, and minimum in Acid Red, the value being 18μg, among 8 dyes (Amaranth, New Coccine, Tartrazine, Sunset Yellow FCF, Erythrosin, Acid Red, Brilliant Blue FCF, and Indigo Carmine) mentioned in the text. 3) As the eluate for separating dyes from the adsorbent (Sephadex), equal volume mixture of 2N-Sulfuric acid and 50% Isopropanol was most excellent. 4) As to the eluate for Brilliant Blue FCF, the mixture of 2N-Sulfuric acid and 50% Isopropanol was most excellent when the concentration of the extract was less than 0.008%, and on the other hand, when it was below 0.03% the mixture of 2M-Acetic acid-Sodium acetate Buffer and 50% Isopropanol was better because this stopped eluation of the accompanying dyes.
岐阜聖徳学園大学の論文
1982-03-31