子宮腺筋症における異所性内膜細胞の特性

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We are presently investigating the involvement of matrix metalloproteinase (MMP-7, MMP-2 and MMP-9) in the invasive ability of human adenomyosis. A collagen gel invasion assay showed that cell cultures from uterine an adenocarcinoma cell line were highly invasive, those from adenomyosis were moderately invasive, those from normal uterine stroma had some invasive ability and those from normal uterine gland had no invasive ability. In situ zymography demonstrated that gelatinase activity was present in adenomyosis and uterine adenocarcinoma, whereas no gelatinase activity was present in normal uterine endometrium. Immunohistochemical study showed that immunoreactive MMP-7 was present on the glandular cells of uterine adenocarcinoma, adenomyosis and normal uterine endometrium, but not on the stromal cells of these tissues. Higher amounts of immunoreactive MMP-7 were present in adenocarcinoma and adenomyosis than in normal uterine endometrium in proliferative phase, whereas immunoreactive MMP-7 was absent in normal uterine endometrium in secretory phase. Immunoreactive MMP-2 and MMP-9 were present in both glandular cells and stromal cells in uterine adenocarcinoma and adenomyosis, whereas those in normal uterine endometrium were weakly expressed in stromal cells whether in the proliferative or secretory phase. An in situ hybridization study showed that MMP-7 mRNA intracellular expression was different according to the tissues used: that in uterine adenocarcinoma cells it was homogenous in cytoplasm; that in adenomyosis it was homogenous in cytoplasm in some cells and was localized at the apical site in other cells, and that in normal uterine endometrium it was localized at the apical site of the cells. The expressions of MMP-2 and MMP-9 mRNAs by in situ hybridization were also characteristic according to the tissues used: MMP-2 and MMP-9 mRNAs in uterine adenocarcima cells were present both in glandular cells and stromal cells; those in adenomyosis were expressed in the stromal cells adjacent to the glandular cells; those in normal endometrium were weakly expressed both in proliferative and secretory phase. The present study is compatible with the idea that the invasive ability of adenomyosis is associated with the glandular expression of MMP-7 and the stromal expression of MMP-2 and MMP-9.
札幌医科大学の論文
1999-06-01

子宮腺筋症における異所性内膜細胞の特性

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