Consensus structure in translation inisiation region in escherichia principal component analysis

概要

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Three major structural features, which may be involved in translation initiation, are drawn from examination of similarity of occurrences of 64 kinds of trinucleotidds (called triplets) in translation initiation regions (TIRs) of Escherichia coli by principal component analysis. (1) In the vicinity of 11-base upstream of translation initiation sites (TISs), there are many 3-purine triplets (especially AGG, GGA, and GAG) except AAA, and extremely few C-rich triplets. This suggests that most of the 3-purine triplets have no C-rich triplets to be partners to form secondary structures, and therefore can go to access to ribosomes. (2) Three triplets (AAA, UAA, and UUU) are generally desirable in the whole region of TIS. The triplet, UUU, is desirable in upstream rather than downstream regions of TIS. These structures may play a role of constructing background structure in TIR. (3) Only two triplets (UAA and UGA) corresponding to the termination codons are desirable in the shifted reading frames downstream of TIS, suggesting that release factors may be also involved in translation initiation because only the two triplets correspond to these factors.
山形大学の論文
1994-01-31