<トピック>糖鎖生物学から糖鎖工学へ(新工学部記念号)

概要

論文の詳細を見る
Glycoproteins are biopolymers that contain one or more oligosaccharide chain covalently linked to a protein. During the past 15 years, it has been generally accepted that the asparagine-linked oligosacharide of glycoprotein is crucial for the function of glycoprotein. Therefore in the biotechnology, many useful human glycoproteins [ie. erythropoietin, tissue plasminogen activator, interferon (β and γ), etc.] are produced by transfecting each human corresponding gene to mammalian cells that have the machinery to synthesize asparagine-linked oligosacoharide. In the present paper, our recent studies on the biosynthesis of lipid-linked oligosaccharide which is the precursor of the asparagine-linked oligosaccharide of glycoprotein were described. We have succeeded in the cloning of human genes of the GDP-mannose dependent mannosyltransferases which are involved in the first half of biosynthesis of biosynthesis of the lipid-linked oligosaccharide. This approach may enable us, not only to clarify the regulation of the biosynthesis of lipid-linked oligosaccharide, but also to apply for the enzymatic synthesis of functional oligosaccharide, and for the diagnosis of human congenital disorders of glycosylation (CDG).
東海大学の論文
2002-03-31