ヒト・マンノース転移酵素複合体の全容解明(2005年度学部等研究教育補助金採択者報告)

概要

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The biosynthesis of the eukaryotic N-glycan precursor, lipid-linked oligosaccharide (LLO), is carried out by a series of glycosyltransferases localized on the rough endoplasmic reticulum (rER) membrane. In the early assembly process, five mannosyltransferase activities are required, which are carried out by three mannosyltransferases. To analyze the physical interaction among them in human, the yeast split-ubiquitin system was applied, which can detect protein-protein interaction on the rER membrane in vivo. Three human mannosyltransferase genes (Hmat-1, Hmat-3 and Hmat-5) were subcloned into a bait or prey vector, and introduced into the yeast NMY51 strain (host cell). Cotransformants with bait and prey constructs were assayed for reporter expression indicating physical interaction. In this assay, three types of interaction were revealed.
東海大学の論文