<原著>ウイルス感染時の表皮角化細胞でのinterleukin-6産生調節機構 : ムンプス感染表皮角化細胞を用いた研究

概要

論文の詳細を見る
Interleukin-6 in epidermal keratinocytes has not been fully elucidated. Human trichilemmoma cell line, K-TL-1 cell line, was used to analyze the presence of both messenger ribonucleic acid (mRNA) transcripts of interleukin-6 (IL-6) and IL-6 protein. Whether IL-6 was synthesized and secreted by the attack of mumps virus (MV), measles virus (MeV) and rubella virus (RV) was determined. Of the three viruses, only MV enhanced IL-6 mRNA expression and production of IL-6 protein in K-TL-1 cell line. The addition of the MV particle alone into the culture media of K-TL-1 cells expressed IL-6 mRNA. The expression of IL-6 mRNA was enhanced at 15 minutes, 30 minutes, 1 and 2 hours in K-TL-1 cell line, but the expression of IL-6 mRNA returned to the steady-state levels at 6,24,48 and 72 hours. The expression of IL-6 mRNA in K-TL-1 cell line was completely inhibited by the addition of 5 μl/ml of polyclonal antibody to MV. These findings show that the binding of MV with K-TL-1 cell certainly causes the expression of IL-6 mRNA.
近畿大学の論文
1992-03-25