<原著>無血清培養系におけるgranulocyte colony-stimulating factorの急性骨髄性白血病細胞に対する影響

概要

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The response of human acute myelogenous leukemia (AML) cells to recombinant human granulocyte colony-stimulating factor (rhG-CSF) was investigated under serum-free culturing conditions. This could be done by using ^3H-thymidine (^3H-TdR) incorporation, leukemic colony forming units (L-CFU), cell cycle and receptor assay. The ^3H-TdR incorporation of AML cells increased 28.9% and the number of L-CFU also increased 50.0% as compared to controls which cultured AML cells without rhG-CSF. The mean ratio of AML cells in the S-phase without rhG-CSF was 8.8%, and it increased to 16.3% after 48 hours of culturing. The ratio of cells in the S-phase which had been stimulated by rhG-CSF for 48 hours increased 25.3%. The proliferation of AML cells in the S-phase was observed in 8 of 11 cases (72.7%). The corresponding ratio of ^3H-TdR incorporation and L-CFU of AML cells was 58.3%. The equal to S-phase ratio of cell cycle and ^3H-TdR incorporation was noted in 10 of 11 cases (90.9%), and that of L-CFU and cell cycle was seen in 7 of 10 cases (70.0%). The G-CSF receptor was detected in all cases. The data indicated that rhG-CSF caused a reaction in 44.7% of the AML cases by using these 3 assays. Therefore, the response of myelogenous leukemia cells to rhG-CSF can be evaluated by a combination of these methods.
近畿大学の論文
1991-06-25